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ICAM3 (8Z7) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE1383
产品名称
ICAM3 (8Z7) Mouse Monoclonal antibody
类别
常规抗体
基因名称
ICAM3
推荐应用
WB
反应种属
Human,Dog,Monkey
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human ICAM3(NP_002153) produced in HEK293T cell.
稀释度
WB 1:400~4000, IHC 1:150, IF 1:100
预测分子量
56.4kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ICAM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1383)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1383)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1383)

Anti-ICAM3 mouse monoclonal antibody (BD-PE1383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ICAM3 .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1383)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ICAM3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE1383)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3(Cat# BD-PE1383). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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