BD-PE1362-GLUL (6S1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GLUL (6S1) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE1362

产品名称

GLUL (6S1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GLUL

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human GLUL (NP_002056) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IHC 1:50, IF 1:50~100, FCM 1:100

预测分子量

41.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. This gene is expressed during early fetal stages, and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in this gene are associated with congenital glutamine deficiency. Several alternatively spliced transcript variants have been found for this gene.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1362)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-GLUL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Figure from citation: Western blot analysis of GLUL protein level by using anti-GLUL antibody in LCC9 cells were treated with 10058-F4 (25 uM) or vehicle for 48 h or transfected with MYC or control siRNA for 48 h. Knockdown of MYC increased GLS/GAC levels and decreased GLUL levels.

Flow cytometric Analysis of Hela cells, using anti-GLUL antibody (BD-PE1362), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunofluorescent staining of HepG2 cells using anti-GLUL mouse monoclonal antibody (BD-PE1362).

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1362)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1362)

Anti-GLUL mouse monoclonal antibody (BD-PE1362) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GLUL .

Flow cytometric Analysis of Jurkat cells, using anti-GLUL antibody (BD-PE1362), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLUL (Cat# BD-PE1362). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.