BD-PE1336-bFGF (FGF2) (1M2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

bFGF (FGF2) (1M2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1336

产品名称

bFGF (FGF2) (1M2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FGF2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

稀释度

WB 1:1000, IHC 1:50, IF 1:100

预测分子量

30.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

BFGF is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BFGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1336)

Anti-BFGF mouse monoclonal antibody (BD-PE1336) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF (Cat# BD-PE1336). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.