Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue within the normal limits using anti-CD23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE1335) (1:500)

Immunofluorescent staining of Jurkat cells using anti-CD23 mouse monoclonal antibody (BD-PE1335, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2 (1:2000).

Immunohistochemical staining of paraffin-embedded mouse spleen tissue within the normal limits using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE1335) (1:500)

Immunohistochemical staining of paraffin-embedded human spleen using FCER2 (CD23) 01, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE1335 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 spleens which show strong membranous and cytoplasmic staining white pulp.

Immunohistochemical staining of paraffin-embedded human tonsil using FCER2 (CD23) 01, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE1335 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tonsils which show strong membranous and cytoplasmic staining however each tonsil has a unique expression pattern in the inner and outer germinal center.