BD-PE1329-ERCC1 (11M17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ERCC1 (11M17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1329

产品名称

ERCC1 (11M17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ERCC1

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Protein expressed in 293T cell transfected with human ERCC1 expression vector

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:2000, IHC 1:50, IF 1:100

预测分子量

32.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of FFPE endometrial carcinoma using heat-induced epitope retrieval HIER at 120°C for 3min with Accel buffer pH8.7, mouse monoclonal antibody anti-ERCC1 was used at 1ug/mL. Strong nuclear stain in tumor cells.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Immunofluorescent staining of HeLa cells using anti-ERCC1 mouse monoclonal antibody (BD-PE1329, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-ERCC1 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE1329 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-ERCC1 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE1329 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody . The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody .

Immunohistochemical staining of FFPE normal adjacent lung from tumor specimen using heat-induced epitope retrieval HIER at 120°C for 3min with Accel buffer pH8.7, mouse monoclonal antibody anti-ERCC1 was used at 1ug/mL. Strong nuclear stain in lung pneumocytes and macrophages.