OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NSE (ENO2) mouse monoclonal antibody (BD-PE1310). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NSE (ENO2) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, BD-PE1310) (1:15000)

Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-NSE (ENO2) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, BD-PE1310) (1:15000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSE (ENO2) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSE(ENO2) antibody (Cat# BD-PE1310)(1:1000).

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NSE (ENO2) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, BD-PE1310) (1:15000)

Immunohistochemical staining of paraffin-embedded Human gastric carcinoma using anti-MUC6 rabbit monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, RM1398) (1:4000)

Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-MUC6 rabbit monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, RM1398) (1:4000)

Immunohistochemical staining of paraffin-embedded Human cervical cancer tissue using anti-P16 (CDKN2A) rabbit monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min, RM1399) (1:9000)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-P16 (CDKN2A) rabbit monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min, RM1399) (1:9000)

Immunohistochemical staining of paraffin-embedded human lung adenocarcinoma tissue using anti-P40 (ΔNp63) rabbit monoclonal antibody (RM1400). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min. (1:35000)