BD-PE1308-EIF4E (9Q16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EIF4E (9Q16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1308

产品名称

EIF4E (9Q16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

EIF4E

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human EIF4E (NP_001959) produced in HEK293T cell.

稀释度

WB 1:500~2000, FCM 1:100

预测分子量

24.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]). [supplied by OMIM]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of permeabilized A549 cells, using anti-EIF4E antibody (BD-PE1308), (Red), compared to an IgG isotype control, (green) (1:100).

Flow cytometric Analysis of Jurkat cells, using anti-EIF4E antibody (BD-PE1308), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF4E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Flow cytometric Analysis of Hela cells, using anti-EIF4E antibody (BD-PE1308), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.