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DPP4 (CD26) (4C9) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE1298
产品名称
DPP4 (CD26) (4C9) Mouse Monoclonal antibody
类别
常规抗体
基因名称
CD26
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human DPP4 (NP_001926) produced in HEK293T cell.
稀释度
WB 1:500, IHC 1:50, IF 1:100, FCM 1:100
预测分子量
88.3kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-DPP4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1298, Dilution 1:50)

Figure from citation: Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells.

Immunohistochemical staining of paraffin-embedded normal human kidney tissue using anti-DPP4 C/N BD-PE1298 mouse monoclonal antibody. HIER Citrate buffer pH6 at 110C for 10 min, anti-DPP4 diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image on left is at 10x magnification and image on the right is at 40x magnificationof area indicated by red arrow on 10x.

Immunohistochemical staining of paraffin-embedded normal human thyroid tissue using anti-DPP4 C/N BD-PE1298 mouse monoclonal antibody. HIER Citrate buffer pH6 at 110C for 10 min, anti-DPP4 diluted to 1:200. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image on left is at 10x magnification and image on the right is at 40x magnification of area indicated by red arrow on 10x.

Figure from citation: Western blot analysis of protein levels of DPP4/8/9 in 293E cells transfected with pcDNA3.1 (Mock), pcDNA3.1-hDPP8 (DPP8) and pcDNA3.1-hDPP9 (DPP9), the purified recombinant human DPP4 protein (rhDPP4) was used as a positive control for the DPP4 antibody, antibodies targeting human DPP4/8/9 (BD-PE1298) and β-actin were purchased from OriGene.

Figure from citation: Immunohistochemistry of DPP4 protein level by using anti-DPP4 antibody in lungs harvested from BALB/c mice at day 3. Dilution: 1:800

Flow cytometric analysis of Hela cells, using anti-DPP4 antibody (BD-PE1298), (Red) compared to a nonspecific negative control antibody (Blue).

Anti-DPP4 mouse monoclonal antibody (BD-PE1298) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP4(Cat# BD-PE1298). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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