OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-beta-catenin mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-beta-catenin very specifically recognizes beta-catenin antigen on OriGene protein microarray chip.

Flow cytometric Analysis of Hela cells, using anti-CTNNB1 antibody (BD-PE1286), (Red), compared to a nonspecific negative control antibody, (Blue).

MDCK cells were treated either with 30mM NaCl as negative control (left) or with GSK-3beta kinase inhibor 30mM LiCl (right) for 48 hrs. Anti-beta-catenin monoclonal antibody was used for immunofluorescent staining.

Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-CTNNB1 4 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1286 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-CTNNB1 4 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1286 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody .

Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using anti-CTNNB1 4 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1286 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1 (1:500).

Immunohistochemical staining of paraffin-embedded mouse brain tissue using anti-CTNNB1 4 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1286 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CTNNB1 4 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE1286 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western Blot analysis of 10 different human tissue lysates (10ug) by using Anti-β-Catenin monoclonal antibody

Immunohistochemical staining of paraffin-embedded Mouse colon tissue using anti-beta-catenin mouse monoclonal antibody.