BD-PE1271-CRYM (mu Crystallin) (13L19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CRYM (mu Crystallin) (13L19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1271

产品名称

CRYM (mu Crystallin) (13L19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CRYM

推荐应用

反应种属

Human,Rat,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CRYM (NP_001879) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

33.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1271)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1271)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CRYM monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1271)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1271)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1271)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYM antibody (BD-PE1271), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1271)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CRYM monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Anti-CRYM mouse monoclonal antibody (BD-PE1271) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYM .

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CRYM monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYM(Cat# BD-PE1271). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.