BD-PE1262-CRABP2 (9D10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CRABP2 (9D10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1262

产品名称

CRABP2 (9D10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CRABP2

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CRABP2 (NP_001869) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

15.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CRABP2 mouse monoclonal antibody (BD-PE1262). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-CRABB2 mouse monoclonal antibody at 1:200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE1262 requires HIER with citrate pH 6.0 at 110°C for 3min using pressure chamber/cooker. The composit of 3 ovarain tumors shows strong nuclear, cytoplasmic, and mebraneous staining in the tumor cells

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-CRABB2 mouse monoclonal antibody at 1:200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE1262 requires HIER with citrate pH 6.0 at 110°C for 3min using pressure chamber/cooker. The composit of 3 ovarain tumors shows strong nuclear, cytoplasmic, and mebraneous staining in the tumor cells.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CRABP2 mouse monoclonal antibody. (BD-PE1262; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)