BD-PE1236-CDK2 (17C11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CDK2 (17C11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1236

产品名称

CDK2 (17C11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CDK2

推荐应用

反应种属

Human,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.

稀释度

WB 1:500~2000, IF 1:100, FCM 1:100

预测分子量

33.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK2 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK2 antibody (BD-PE1236), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-CDK2 antibody, (Red), compared to PBS (green) and a nonspecific negative control antibody, (Blue) (1:100).

Immunofluorescent staining of HeLa cells using anti-CDK2 mouse monoclonal antibody (BD-PE1236).

Anti-CDK2 mouse monoclonal antibody (BD-PE1236) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK2 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 14 cell lines by using anti-CDK2 monoclonal antibody (1:500).