Living HEK293T cells transfected with either plasmid (red) or empty vector (blue) were immunostained by anti-CDH2 antibody (BD-PE1230) or isotype control antibody (green), and then analyzed by flow cytometry (1:50).

Western blot analysis of extracts (35ug) from cell line and tissues by using anti-CDH2 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded of human thyroid carcinoma using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the tumor cells

Immunohistochemical staining of paraffin-embedded of human kidney using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining in the kidney tubules

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CDH2 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE1230 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western blot analysis of extracts (35ug) from 6 cell lines by using anti-CDH2 monoclonal antibody (1:500).

Western blot of mouse tissue lysates (20ug) from Brain. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-CDH2 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE1230 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded of human carcinoma of the lung using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows the tumor cells with membraneous and cytoplasmic staining.

Immunohistochemical staining of paraffin-embedded of human lymph node using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows cells of the lymph node are negative. Positive staining seen in the endothelial cells, mostly membraneous with weak cytoplasmic.

Immunohistochemical staining of paraffin-embedded of human ovarian carcinoma using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the tumor cells

Immunohistochemical staining of paraffin-embedded of human endometrium using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the endometrial cells.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH2 mouse monoclonal antibody (1:1000).

Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-CDH2 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE1230 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded of human pancreas using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows gladular cells of the pancreas membraneous and cytoplasmic staining cells.

Immunofluorescent staining of HeLa cells using anti-CDH2 mouse monoclonal antibody (BD-PE1230, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded of 3 human carcinoma of kidney using anti-CDH2 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. BD-PE1230 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human carcinoma of the kidney all show strong membraneous and cytoplasmic staining in the tumor cells.