BD-PE1221-CD63 (18K10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD63 (18K10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1221

产品名称

CD63 (18K10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD63

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD63 (NP_001771) produced in HEK293T cell.

稀释度

WB 1:2000

预测分子量

25.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of MCF-7 cells, using anti-CD63 antibody (BD-PE1221), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD63 antibody (BD-PE1221), and then analyzed by flow cytometry (1:100).

Flow cytometric Analysis of living A549 cells, using anti-CD63 antibody (BD-PE1221), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (35ug) from MCF7 cell line by using anti-CD63 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD63. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.