Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

Flow Cytometric analysis of viable B-Cell Lymphoma cell line (Raji) using anti-CD19 antibody (BD-PE1204-red histogram), compared to isotype control antibody (anti-DDK TA180144- green histogram), and no antibody control PBS (blue histogram). (CD19 titer 1:20)

Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD19 03 mouse monoclonal antibody at 1:200 dilution of 0.6mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1204 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >40 % of non germinal center cells of tonsil and >90% of the germinal center cells. No staining was seen in the squamous epithelia cells.

Flow Cytometric analysis of viable human peripheral blood mononuclear cells (PBMCs) using anti-human CD19 antibody (BD-PE1204-red histogram), compared to isotype control antibody (anti-DDK - green histogram). (CD19 titer 1:20)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CD19 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Western blot of cell lysates (35ug) from Jurkat. Diluation: 1:500

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (BD-PE1204; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)