OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD2 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-CD2 very specifically recognizes Cd2 antigen on OriGene protein microarray chip.

Flow cytometric analysis of living 293T cells transfected with CD2 overexpression plasmid (Red)/empty vector (Blue) using anti-CD2 antibody (BD-PE1194). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Flow cytometric analysis of living CCRF-CEM cells, using anti-CD2 antibody (BD-PE1194, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Flow cytometric analysis of living Jurkat cells, using anti-CD2 antibody (BD-PE1194, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

IHC staining of FFPE human spleen using anti-CD2 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB. BD-PE1194 requires heat-induced epitope retrieval with Citrate pH6.0. The image shows strong membranous and cytoplasmic staining.

Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE1194) (1:500).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (1:1000).

Immunofluorescent staining of Jurkat cells using anti-CD2 mouse monoclonal antibody (BD-PE1194, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 8µm.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2 (1:500).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE1194) (1:500).

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD2 mouse monoclonal antibody.

IHC staining of paraffin-embedded human tonsil using anti-CD2 mouse monoclonal antibody at 1:200 of 0.6mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1194 requires heat-induced epitope retrieval with Accel pH8.7 at 95-100C 30 minutes or 10 min in pressure cooker. The image shows strong membranous and cytoplasmic staining in >50 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.

Immunohistochemical staining of paraffin-embedded mouse colon tissue within the normal limits using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE1194) (1:500)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CD2 mouse monoclonal antibody.