BD-PE1193-CD2 (15H10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD2 (15H10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1193

产品名称

CD2 (15H10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD2

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD2 (NP_001758) produced in HEK293T cell.

稀释度

WB 1:2000

预测分子量

39.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. [supplied by OMIM, Jul 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of living 293T cells transfected with CD2 overexpression plasmid (Red)/empty vector (Blue) using anti-CD2 antibody (BD-PE1193). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.