BD-PE1147-ATP6V1B1 (17E15) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ATP6V1B1 (17E15) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1147

产品名称

ATP6V1B1 (17E15) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ATP6V1B1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ATP6V1B1 (NP_001683) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

56.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody (BD-PE1147), and then analyzed by flow cytometry.

Flow cytometric Analysis of Jurkat cells, using anti-ATP6V1B1 antibody (BD-PE1147), (Red), compared to a nonspecific negative control antibody , (Blue).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATP6V1B1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1147)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATP6V1B1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1147)

Flow cytometric Analysis of Hela cells, using anti-ATP6V1B1 antibody (BD-PE1147), (Red), compared to a nonspecific negative control antibody , (Blue).

Anti-ATP6V1B1 mouse monoclonal antibody (BD-PE1147) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1(Cat# BD-PE1147). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.