BD-PE1141-ATP5B (7B15) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ATP5B (7B15) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1141

产品名称

ATP5B (7B15) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ATP5F1B

推荐应用

反应种属

Human,Dog,Monkey

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:50, IF 1:100, FCM 1:100

预测分子量

56.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1141, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1141, Dilution 1:50)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1141, Dilution 1:50)

HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal (BD-PE1141), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1141, Dilution 1:50)

Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody (BD-PE1141).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1141, Dilution 1:50)

Flow cytometric analysis of Hela cells, using anti-ATP5B antibody (BD-PE1141), (Red) compared to a nonspecific negative control antibody (Blue).

Anti-ATP5B mouse monoclonal antibody (BD-PE1141) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B .

Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody (BD-PE1141), (Red) compared to a nonspecific negative control antibody (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.