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APEX1 (14N9) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE1130
产品名称
APEX1 (14N9) Mouse Monoclonal antibody
类别
常规抗体
基因名称
APEX1
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 1-242 of human APEX1 (NP_001632) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100
预测分子量
35.4kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2a
背景介绍
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-APEX1 antibody (BD-PE1130), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Anti-APEX1 mouse monoclonal antibody (BD-PE1130) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APEX1 .

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1130)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APEX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APEX1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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