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PARP1 (19V16) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE1119
产品名称
PARP1 (19V16) Mouse Monoclonal antibody
类别
常规抗体
基因名称
PARP1
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human PARP1 (NP_001609) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150
预测分子量
112.9kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARP1 (Cat# BD-PE1119)(1:2000).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1119) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARP1(Cat# BD-PE1119). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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