BD-PE1083-GCLC (8N11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GCLC (8N11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1083

产品名称

GCLC (8N11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GCLC

推荐应用

反应种属

Human,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.

稀释度

WB 1:200~1000, IHC 1:150

预测分子量

72.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction. [provided by RefSeq, Oct

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GCLC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1083)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1083)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE1083)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCLC(Cat# BD-PE1083). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.