BD-PE1059-EFNA2 (Ephrin A2) (14O12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EFNA2 (Ephrin A2) (14O12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1059

产品名称

EFNA2 (Ephrin A2) (14O12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Ephrin A2

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

21.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human EFNA2 plasmid (hEFNA2), mouse EFNA2 plasmid (mEFNA2) using anti-EFNA2 antibody BD-PE1059(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFNA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFNA2(Cat# BD-PE1059). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Flow cytometric Analysis of Jurkat cells, using anti-EFNA2 antibody (BD-PE1059), (Red), compared to a nonspecific negative control antibody , (Blue).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EFNA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE1059)

Anti-EFNA2 mouse monoclonal antibody (BD-PE1059) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EFNA2 .