OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CTAG1B mouse monoclonal antibody (BD-PE1038). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B (1:500).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Flow cytometric analysis of living 293T cells transfected with CTAG1B overexpression plasmid , Red)/empty vector (Blue) using anti-CTAG1B antibody (BD-PE1038). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Flow cytometric analysis of living NCI-H1299 cells, using anti-CTAG1B antibody (BD-PE1038, Red), compared to an isotype control (green) (1:100).

Flow cytometric analysis of living A375 cells, using anti-CTAG1B antibody (BD-PE1038, Red), compared to an isotype control (green) (1:100).

Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-CTAG1B mouse monoclonal antibody. (BD-PE1038; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)