BD-PE1034-Purified CTAG1B (NY-ESO-1) (19D6) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Purified CTAG1B (NY-ESO-1) (19D6) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1034

产品名称

Purified CTAG1B (NY-ESO-1) (19D6) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CTAG1B

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CTAG1B (NP_001318) produced in HEK293T cell.

稀释度

WB 1:500~1000, IF 1:50~100

预测分子量

17.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Cancer/testis antigens, such as CTAG1B, are expressed in a variety of malignant tumors but soley in testis among normal adult tissues (Yoshida et al., 2006 [PubMed 16596224]). [supplied by OMIM]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CTAG1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunofluorescent staining of A549 cells using anti-CTAG1B mouse monoclonal antibody (BD-PE1034).

Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, BD-PE1034) (1:150)

Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B (1:500).