BD-PE1010-CD68 (14Q7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD68 (14Q7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE1010

产品名称

CD68 (14Q7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD68

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 22-319 of human CD68 (NP_001242)produced in SF9 cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:2000, IHC 1:100~200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot of cell lysates (35ug) from 4 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD68 mouse monoclonal antibody. (BD-PE1010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Western blot analysis of COLO205 cell lysate (35ug) by using anti-CD68 monoclonal antibody. The molecular weight of highly glycosylated CD68 is between 75-110 kDa.

Immunohistochemical staining of paraffin-embedded of 3 human spleen using anti-CD68 50 mouse monoclonal antibody at 1:200 dilution of 0.9mg/mL and detection with Polink2 Broad HRP DAB. BD-PE1010 requires heat-induced epitope retrieval with Accel 3in1 EDTA solution pH8.7 at 95-100C 20 minutes. The composit image of 3 human spleens show all showstrong membranous and cytoplasmic staining mainly in the red pulp region of spleen.

Western blot analysis of MALME3M cell lysate (35ug) by using anti-CD68 monoclonal antibody. The molecular weight of highly glycosylated CD68 is between 75-110 kDa.

Western blot analysis of A498 cell lysate (35ug) by using anti-CD68 monoclonal antibody. The molecular weight of highly glycosylated CD68 is between 75-110 kDa.

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD68 mouse monoclonal antibody (BD-PE1010). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD68 mouse monoclonal antibody. (BD-PE1010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)