OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD40 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE1005) (1:600)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE1005) (1:600)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40 (1:2000).

Immunohistochemical staining of paraffin-embedded cell pellets (The left is Raji and the right is Romas) using anti-CD40 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE1005) (1:600).

Western blot analysis of extracts (10ug) from Raji cell line by using anti-IDO1 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE1005) (1:600)