BD-PE0998-TNFRSF8 (CD30) (13A18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TNFRSF8 (CD30) (13A18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0998

产品名称

TNFRSF8 (CD30) (13A18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

TNFRSF8

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 408-595 of human TNFRSF8(NP_001234) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500~2000, IHC 1:100~300

预测分子量

63.75kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TNFRSF8 (CD30) mouse monoclonal antibody (BD-PE0998). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded Carcinoma of cell pellets (The left is K562 and the right is Jurkat) using anti-TNFRSF8 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE0998) (1:300).

Western blot analysis of extracts (10ug) from K562 cell line by using anti-IDO1 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0998) (1:300)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (CD30) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8 (CD30) (1:2000).

Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0998) (1:300)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0998) (1:300)

Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0998) (1:300)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0998) (1:300)