BD-PE0987-CA12 (5I16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CA12 (5I16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0987

产品名称

CA12 (5I16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CA12

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CA12(NP_001209) produced in HEK293T cell.

稀释度

IHC 1:100~200

预测分子量

36.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot of cell lysates (35ug) from A549. Diluation: 1:500

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CA12 mouse monoclonal antibody. (BD-PE0987; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CA12 mouse monoclonal antibody. (BD-PE0987; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-CA12 mouse monoclonal antibody. (BD-PE0987; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells using anti-CA12 mouse monoclonal antibody (BD-PE0987, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CA12 mouse monoclonal antibody. (BD-PE0987; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)