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IRF3 (14M8) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0971
产品名称
IRF3 (14M8) Mouse Monoclonal antibody
类别
常规抗体
基因名称
IRF3
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 27-169 of human IRF3(NP_001184053) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0971) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0971) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0971) (1:150)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0971) (1:150)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0971) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).

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