BD-PE0967-BID (15X19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BID (15X19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0967

产品名称

BID (15X19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BID

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.

稀释度

WB 1:400~4000, IHC 1:150

预测分子量

21.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0967)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0967)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0967)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BID monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0967)

Immunofluorescent staining of HeLa cells using anti-BID mouse monoclonal antibody (BD-PE0967).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.