BD-PE0937-Purified BIRC5/Survivin (11S14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Purified BIRC5/Survivin (11S14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0937

产品名称

Purified BIRC5/Survivin (11S14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BIRC5

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human BIRC5(NP_001159) produced in HEK293T cell.

稀释度

WB 1:2000, IF 1:100, FCM 1:100

预测分子量

16.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 1 cell line by using anti-BIRC5 monoclonal antibody (1:200).

Flow cytometric analysis of living 293T cells transfected with BIRC5 overexpression plasmid , Red)/empty vector (Blue) using anti-BIRC5 antibody (BD-PE0937). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Anti-BIRC5 mouse monoclonal antibody (BD-PE0937) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIRC5 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.