产品名称
TRIB3 Rabbit Polyclonal Antibody
基因名称
TRIB3 C20orf97 NIPK SKIP3 TRB3
蛋白名称
Tribbles homolog 3 (TRB-3) (Neuronal cell death-inducible putative kinase) (SINK) (p65-interacting inhibitor of NF-kappa-B)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q96RU7
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96RU7/entry
Mouse Swissprot No.
Q8K4K2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K4K2
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q9WTQ6
免疫原
Synthesized peptide derived from part region of human protein
特异性
TRIB3 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014],
组织表达
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
功能
caution:The role of this protein in Akt activation has been demonstrated by Du et al (PubMed:12791994) for the mouse ortholog but Iynedjian (PubMed:15469416) has not been able to reproduce the result in rat hepatocytes.,function:Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.,induction:By hypoxia, TNF and by nutrient starvation. Expression is PI 3-kinase and/or NF-kappa-B-dependent.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with AKT1, AKT2, ATF4, MAP2K1 and MAP2K7.,tissue specificity:Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
