BD-PE0927-ALDH4A1 (6J15) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ALDH4A1 (6J15) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0927

产品名称

ALDH4A1 (6J15) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ALDH4A1

推荐应用

反应种属

Human,Dog,Rat,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 1-503 of human ALDH4A1 (NP_001154976) produced in E.coli.

稀释度

WB 1:500, IHC 1:500

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ALDH4A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0927) (1:500)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ALDH4A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0927) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH4A1 (Cat# BD-PE0927)(1:500).

Western blot analysis of extracts (35ug) from 10 different cell lines by using anti-ALDH4A1 monoclonal antibody (1:500).