BD-PE0897-AFP (8U14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AFP (8U14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0897

产品名称

AFP (8U14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

AFP

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100, IF 1:100

预测分子量

66.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-AFP mouse monoclonal antibody (BD-PE0897). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunofluorescent staining of HepG2 cells using AFP mouse monoclonal antibody (BD-PE0897, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded human placenta using anti-AFP 6 mouse monoclonal antibody at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. BD-PE0897 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous, cytoplasmic and rare nuclear protein in the trophoblast cells. This protein is also seen as secreted protein.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody .

Immunohistochemical staining of paraffin-embedded human embryonic liver tissue using anti-AFP mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min in pressure chamber/cooker. BD-PE0897 diluted 1:100 and detection shown with HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in the hepatocytes.