BD-PE0890-AFP (alpha-fetoprotein) (3P16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AFP (alpha-fetoprotein) (3P16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0890

产品名称

AFP (alpha-fetoprotein) (3P16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

alpha 1 Fetoprotein

推荐应用

反应种属

Human,Dog,Monkey

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

稀释度

WB 1:200~500, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

66.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0890)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AFP antibody (BD-PE0890), and then analyzed by flow cytometry.

Anti-AFP mouse monoclonal antibody (BD-PE0890) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP .

Flow cytometric Analysis of Jurkat cells, using anti-AFP antibody (BD-PE0890), (Red), compared to a nonspecific negative control antibody , (Blue).

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-AFP monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP(Cat# BD-PE0890). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.