BD-PE0850-alpha-actinin (Actinin alpha 1) (16U3) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

alpha-actinin (Actinin alpha 1) (16U3) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0850

产品名称

alpha-actinin (Actinin alpha 1) (16U3) Mouse Monoclonal antibody

类别

常规抗体

基因名称

alpha Actinin

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.

稀释度

WB 1:2000, IHC 1:50, IF 1:100

预测分子量

102.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0850)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0850)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0850)

Immunofluorescent staining of HeLa cells using anti-ACTN1 mouse monoclonal antibody (BD-PE0850).

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0850)

Anti-ACTN1 mouse monoclonal antibody (BD-PE0850) immunofluorescent staining of Hela cells transiently transfected by pCMV6-ENTRY ACTN1 .

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0850)

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1(Cat# BD-PE0850). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.