BD-PE0795-TOP2A (17S7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TOP2A (17S7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0795

产品名称

TOP2A (17S7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

TOP2A

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 1100-1531 of human TOP2A(NP_001058) produced in E.coli.

稀释度

IHC 1:200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-TOP2A mouse monoclonal antibody. (BD-PE0795; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

Immunofluorescent staining of HeLa cells using anti-TOP2A mouse monoclonal antibody (BD-PE0795, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. (BD-PE0795; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human colon cancer using anti-TOP2A 45 mouse monoclonal antibody at 1:333 with Polink2 Broad HRP DAB detection kit (; heat-induced epitope retrieval with Accel buffer (B22C, pH8.5)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody. (BD-PE0795; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)