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TF (Transferrin) (6M3) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0791
产品名称
TF (Transferrin) (6M3) Mouse Monoclonal antibody
类别
常规抗体
基因名称
Transferrin
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human TF(NP_001054) produced in HEK293T cell.
稀释度
IF 1:100, FCM 1:100
预测分子量
75.1kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody (BD-PE0791), and then analyzed by flow cytometry.

Anti-TF mouse monoclonal antibody (BD-PE0791) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF .

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TF (Cat# BD-PE0791)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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