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SULT1A1 (17B12) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0780
产品名称
SULT1A1 (17B12) Mouse Monoclonal antibody
类别
常规抗体
基因名称
SULT1A1
推荐应用
WB
反应种属
Human,Monkey
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human SULT1A1 (NP_001046) produced in HEK293T cell.
稀释度
WB 1:500~2000, IHC 1:150, FCM 1:100
预测分子量
34.0kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Multiple alternatively spliced variants that encode two isoforms have been identified for this gene. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SULT1A1 antibody (BD-PE0780), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SULT1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0780)

Flow cytometric Analysis of Jurkat cells, using anti-SULT1A1 antibody (BD-PE0780), (Red), compared to a nonspecific negative control antibody , (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT1A1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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