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NGDN (18V3) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0773
产品名称
NGDN (18V3) Mouse Monoclonal antibody
类别
常规抗体
基因名称
NGDN
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human NGDN (NP_001036100) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150
预测分子量
35.7kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
Neuroguidin is an EIF4E (MIM 133440)-binding protein that interacts with CPEB (MIM 607342) and functions as a translational regulatory protein during development of the vertebrate nervous system (Jung et al., 2006 [PubMed 16705177]). [supplied by OMIM, Mar 2008]. Transcript Variant: This variant (2) retains an intron, differing in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AL050003.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025081, ERS025082 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NGDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0773) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NGDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NGDN (1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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