BD-PE0762-SIRPA (11Z9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SIRPA (11Z9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0762

产品名称

SIRPA (11Z9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SIRP alpha

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SIRPA (NP_001035111) produced in HEK293T cell.

稀释度

WB 1:2000

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE0762) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE0762) (1:150)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE0762) (1:150)

Flow cytometric analysis of living 293T cells transfected with SIRPA overexpression plasmid , Red)/empty vector (Blue) using anti-SIRPA antibody (BD-PE0762). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE0762) (1:150)

Flow cytometric analysis of living ACHN cells, using anti-SIRPA antibody (BD-PE0762, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE0762) (1:150)

Flow cytometric analysis of living A375 cells, using anti-SIRPA antibody (BD-PE0762, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Flow cytometric analysis of living SK-MEL-28 cells, using anti-SIRPA antibody (BD-PE0762, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRPA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRPA (1:2000) (Cat# BD-PE0762). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.