BD-PE0745-CTLA4 (11J1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CTLA4 (11J1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0745

产品名称

CTLA4 (11J1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CTLA4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CTLA4 (NP_001032720) produced in HEK293T cell.

稀释度

WB 1:500

预测分子量

15kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either CTL4 plasmid (red) or empty vector control plasmid (blue).The cells were immunostained by anti-CTLA4 antibody and analyzed by flow cytometry (1:100).

Flow cytometric analysis of CTLA4 expression on stimulated human peripheral blood mononuclear cells (PBMC). Unstimulated PBMC, (a.left, b.blue), or 10ug/ml Phytohemagglutinin-stimulated (3 days) PBMC, (a.right, b.red), were stained with Anti-Human CTLA4 antibody (BD-PE0745) (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTLA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTLA4 (Cat# BD-PE0745)(1:500). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of human peripheral blood mononuclear cells (PBMCs) by using anti-CTLA4 monoclonal antibody. Left: Unstimulated PBMC. Right: PBMC stimulated with 10ug/ml Phytohemagglutinin for 3 days (1:100).