BD-PE0734-RRM1 (6U6) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RRM1 (6U6) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0734

产品名称

RRM1 (6U6) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RRM1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 541-792 of human RRM1 (NP_001024) produced in E.coli.

稀释度

IHC 1:200

预测分子量

89.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RRM1 mouse monoclonal antibody. (BD-PE0734; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RRM1 mouse monoclonal antibody. (BD-PE0734; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RRM1 mouse monoclonal antibody. (BD-PE0734; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunofluorescent staining of HeLa cells using anti-RRM1 mouse monoclonal antibody (BD-PE0734, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RRM1 mouse monoclonal antibody. (BD-PE0734; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)