Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0696)

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-VEGF monoclonal antibody (1:200).

HEK293T cells were transfected with the overexpression plasmids of 9 VEGF isoforms (from left to right:VEGF206; VEGF189; L-VEGF165; VEGF165; L-VEGF121; VEGF iso18; VEGF183; VEGF145; L-VEGF189) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (Cat# , 1:1000) or anti-VEGFA mouse monoclonal antibody. (Cat# BD-PE0696, 1:500)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0696)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0696)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0696)

Anti-VEGF mouse monoclonal antibody (BD-PE0696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGF .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF (Cat# BD-PE0696).