BD-PE0695-VEGFA (VEGF) (3P5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

VEGFA (VEGF) (3P5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0695

产品名称

VEGFA (VEGF) (3P5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

VEGFA

推荐应用

反应种属

Human,Dog

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.

稀释度

WB 1:2500~5000

预测分子量

23.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

VEGF is a member of the PDGF/VEGF growth factor family that is often found as a disulfide linked homodimer. It is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in VEGF gene have been associated with proliferative and nonproliferative diabetic retinopathy.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VEGF mouse monoclonal antibody. (BD-PE0695)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VEGF monoclonal antibody.

HEK293T cells were transfected with the overexpression plasmids of 9 VEGF isoforms (from left to right:VEGF206, ; VEGF189, ; L-VEGF165, ; VEGF165, ; L-VEGF121, ; VEGF iso18, ; VEGF183, ; VEGF145, ; L-VEGF189, ) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (1:1000) or anti-VEGFA mouse monoclonal antibody. (BD-PE0695, 1:500)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VEGF mouse monoclonal antibody. (BD-PE0695)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF.