OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD74 mouse monoclonal antibody (BD-PE0688). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100)

Immunohistochemical staining of paraffin-embedded NCI-H2228 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0688) (1:1600)

Immunohistochemical staining of paraffin-embedded Raji cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0688) (1:1600)

Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD74 1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0688) (1:800).

Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using anti-CD74 1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0688) (1:800).

Immunohistochemical staining of paraffin-embedded HL-60 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0688) (1:1600)

Immunohistochemical staining of paraffin-embedded MDA-MB-231 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0688) (1:1600)

Immunohistochemical staining of paraffin-embedded LoVo cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0688) (1:1600)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD74 (1:500).

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD74 1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0688) (1:800)

Immunohistochemical staining of paraffin-embedded Ramos cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE0688) (1:1600)

Immunohistochemical staining of paraffin-embedded mouse colon tissue using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0688) (1:800).

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 2.5 min, BD-PE0688) (1:1600)