BD-PE0664-MUC1/EMA (8L1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MUC1/EMA (8L1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0664

产品名称

MUC1/EMA (8L1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

EMA

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MUC1 (NP_001018016) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100

预测分子量

25.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MUC1 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-MUC1 very specifically recognizes MUC1 antigen on OriGene protein microarray chip (1:100).

Immunohistochemical staining of paraffin-embedded human colon cancer using anti-MUC1 (EMA) mouse monoclonal antibody at 1:200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE0664 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tumors which show strong membranous and secreted protein however >75 % tumor cells are negative for cytoplasmic staining.

Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-MUC1 (EMA) mouse monoclonal antibody at 1:200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE0664 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tumors which show strong membranous and weak cytoplasmic staining in >75 % tumor cells.

Immunohistochemical staining of paraffin-embedded human stomach carcinoma using anti-MUC1 (EMA) mouse monoclonal antibody at 1:200 dilution of1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE0664 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tumors which show strong membranous and cytoplasmic staining in >75 % tumor cells.

Immunofluorescent staining of MCF-7 cells using anti-MUC1/EMA mouse monoclonal antibody (BD-PE0664, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-MUC1 monoclonal antibody (1:500).