BD-PE0644-MAGEA4 (1O5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MAGEA4 (1O5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0644

产品名称

MAGEA4 (1O5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MAGEA4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MAGEA4(NP_001011550) produced in HEK293T cell.

稀释度

WB 1:4000, IHC 1:150

预测分子量

34.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from serum-free cell culture supernatant by affinity chromatography Protein A/G (from mouse hybridoma)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0644)

Flow cytometric analysis of fixed and permeabilized NCI-H1299 cells, using anti-MAGEA4 antibody (BD-PE0644, Red), compared to an isotype control (green) (1:100).

Flow cytometric analysis of living A375 cells, using anti-MAGEA4 antibody (BD-PE0644, Red), compared to an isotype control (green) (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4(Cat# BD-PE0644). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-MAGEA4 monoclonal antibody (1:500).