BD-PE0643-MAGEA4 (5E17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MAGEA4 (5E17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0643

产品名称

MAGEA4 (5E17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MAGEA4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MAGEA4(NP_001011550) produced in HEK293T cell.

稀释度

WB 1:500, IHC 1:150, IF 1:100

预测分子量

34.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0643)

Immunohistochemical staining of paraffin-embedded untransfected MAGEA4 CytoSections using anti-MAGEA4 antibody BD-PE0643 mouse monoclonal antibody. HIER Citrate buffer pH6 at 110C for 10 min, anti-MAGEA4 was diluted to 1:1000. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0643)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0643)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0643)

Anti-MAGEA4 mouse monoclonal antibody (BD-PE0643) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAGEA4 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.